![]() ![]() The accession number for the raw and processed HeLa BRIC-Seq data and HeLa ribosome sequencing data used in this study is NCBI Gene Expression Omnibus GSE102113. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The accession number for the raw data and processed data files for the CHO 5-EU-Seq and HeLa tRNA-Seq results reported in this study is NCBI Gene Expression Omnibus GSE138292. Received: DecemAccepted: JanuPublished: February 13, 2020Ĭopyright: © 2020 Forrest et al. PLoS ONE 15(2):Įditor: Yoon Ki Kim, Korea University, REPUBLIC OF KOREA Together, these results raise the possibility that intracellular tRNA and amino acid levels interplay to mediate coupling between translational elongation and mRNA degradation rate in mammals.Ĭitation: Forrest ME, Pinkard O, Martin S, Sweet TJ, Hanson G, Coller J (2020) Codon and amino acid content are associated with mRNA stability in mammalian cells. Accordingly, genes of similar physiological function appear to coordinate their mRNA stabilities in part through codon and amino acid content. The impact of codon and amino acid identity on mRNA decay appears to be associated with underlying tRNA and intracellular amino acid concentrations. Surprisingly, however, we also observe that the amino acid content of a gene is an additional determinant correlating with transcript stability. ![]() In agreement with previous studies, we observed that synonymous codon usage impacts mRNA stability in mammalian cells. Herein we characterize the contribution of coding sequence towards mRNA decay in human and Chinese Hamster Ovary cells. ![]() Importantly, however, the degradation machinery is highly conserved, thus it seems logical that mammalian transcript half-lives would also be strongly influenced by coding determinants. General principles governing mRNA stability are poorly understood in mammalian systems. In yeast and other model organisms, codon identity is a powerful determinant of transcript stability, contributing broadly to impact half-lives. Messenger RNA (mRNA) degradation plays a critical role in regulating transcript levels in the cell and is a major control point for modulating gene expression. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |